Coding

Part:BBa_K2984056:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-Psad-HygR-Rbcs2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1623
    Illegal NgoMIV site found at 1731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Hygromycin resistance desinged for C.reinhardti transformation and selection or further cloning into Level 2 backbone with MoClo Standard



Source

The construct consist of the four different Level 0 parts which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo standard


References